Correction of Absorption Spectra of a pH-Sensitive Dye for Intracellular pH Estimation in Smooth Muscle.
نویسندگان
چکیده
منابع مشابه
Measurement of intracellular pH of skeletal muscle with pH-sensitive glass microelectrodes.
We used three methods to examine the relationship among intracellular pH, transmembrane potential, and extracellular pH. Single-barreled electrodes permitted the determination of resting potential and intracellular pH with a minimum of cellular injury. Double-barreled electrodes, which incorporated a reference as well as a pH-sensitive electrode in a single tip, facilitated the direct measureme...
متن کاملIntracellular calibration of a pH-sensitive dye in isolated, perfused salamander proximal tubules
We evaluated the dye 4',5'-dimethyl-5-(and -6-) carboxyfluorescein (Me2CF) for determining the intracellular pH(pHi) of isolated, perfused proximal tubules of the salamander. The intracellular absorbance spectrum, corrected for the intrinsic absorbance of the tubule, was obtained once per second. The dye was incorporated into tubule cells by exposing them to the membrane-permeable precursor 4',...
متن کاملA review of the actions and control of intracellular pH in vascular smooth muscle.
OBJECTIVE This review is an account of the physiological issues involved in the effects of pH on vascular smooth muscle tone. The following criteria were considered when reviewing the literature: (i) the type of smooth muscle, i.e. either tonic or phasic, (ii) the source of the smooth muscle i.e. pulmonary, systemic, large artery, resistance artery, vein or cell line, (iii) the effects of chang...
متن کاملpH-Responsive quantum dots (RQDs) that combine a fluorescent nanoparticle with a pH-sensitive dye.
A quantum dot conjugated to a dye through an experimentally simple process of self-assembly exhibits an enhanced emission when the dye is attached, and this effect is pH-sensitive.
متن کاملHigh-throughput phagocytosis assay utilizing a pH-sensitive fluorescent dye.
We describe a development of a novel high-throughput phagocytosis assay based on a pH-sensitive cyanine dye, CypHer5E, which is maximally fluorescent in an acidic environment. This dye is ideally suited for the study of phagocytosis because of the acidic conditions generated in the intracellular phagocytic vesicles after particle uptake. Use of CypHer5E-labeled particles results in greatly redu...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The Japanese Journal of Physiology
سال: 1993
ISSN: 0021-521X
DOI: 10.2170/jjphysiol.43.103